By M. Ahearne, Alicia J. El Haj, S. Rauz (auth.), Alicia El Haj, Dan Bader (eds.)
This quantity offers chosen peer-reviewed papers of the eighth foreign convention on phone & Stem mobile Engineering (ICCM) 2010 in Dublin. The contributions are written through prime scientists in phone and Stem phone Engineering and the subjects of the papers contain:
Computational telephone Mechanics
Experimental concepts in cellphone Mechanics
Molecular and cellphone Imaging
Cell Matrix Interactions
Mechanotransduction and telephone mechanics
Stem cellphone area of interest
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Extra info for 8th International Conference on Cell & Stem Cell Engineering (ICCE)
23–25, 2011. com 24 B. Hu et al. Hs99999901_m1;) and STRATGENE Mx3005P QPCR system was used. The cycle condition is set according to the manufacturer’s protocol. Statistical Analysis One way ANOVA was used for results statistics analysis. 05 was considered significant. III. RESULTS AND ANALYSIS A. Immnuofluorescence Staining Images a b b Fig. 05). 05) c c C. Real Time RT-PCR d d e e Compared with untreated cells, after 3 hrs further culture, mRNA of cells stimulated via PDGFRα conjugated MNPs were upregulated; after 24 hrs further culture, mRNA of cells stimulated via both PDGFRα and PDGFRβ conjugated MNPs were upregulated.
We have previously remedied this by adding negatively charged macromolecules, which drives the conversion of procollagen to collagen and thus enhances collagen deposition dramatically [12, 13]. We have taken this technology further by decellularising these bioassembled matrices to create cell-free supports that allows for increased population doubling rate and better retention of differentiation capacity in MSCs and hESCs. II. I. INTRODUCTION Standard culture of MSCs on TCPS ages the cells fast and decrease their self renewal [1, 2].
7. 8. 9. 10. 11. 12. 13. ACKNOWLEDGEMENTS Funding was provided by IRCSET, and the President of Ireland Young Researcher Award (08/Y15/B1336). REFERENCES 1. 2. Benya P, Shaffer J (1982) Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels. Cell 30: 215-224 Buschmann M, Gluzband Y, Grodzinsky A et al. (1992) Chondrocytes in agarose culture synthesize a mechanically functional extracellular matrix. Journal of Orthopaedic Research 10: 745-758 14.